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Binding of FAK and HIF-1 during mechanoperception
Patricia Sanz-Ramos1, Iñigo Izal-Azcárate1
1Laboratory for Orthopedic Research, University of Navarra, School of Medicine, Pamplona, Spain.

Article ID: 100002C06PR2014
doi:10.5348/c06-2014-2-SR-2

Address correspondence to:
Iñigo Izal-Azcárate
Laboratory for Orthopaedic Research, School of Medicine
Ed. Los Castaños, Irunlarrea s/n, 31008
Pamplona, Navarra
Spain
Phone: 00 34 48 425600 (6403)
Fax: 00 34 48 425649
Email: inizal@me.com

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How to cite this article
Sanz-Ramos P, Izal-Azcárate I. Binding of FAK and HIF-1 during mechanoperception. Edorium J Cell Biol 2014;1:12–15.


Abstract
Aims: We have suggested the interaction of HIF-1a and FAK, as a mechanism for the influence of low oxygen concentrations in the mechanoperception in chondrocytes. We aimed here, to analyze the binding capacity for HIF-1a and FAK in protein extracts from rat chondrocytes in hypoxia.
Methods: We have used protein extracts from chondrocytes subjected to changes in substrate stiffness for 30 minutes used them for immunoprecipitation and pull down assays. Results: We show the binding capacity for FAK and HIF-1a and demonstrate that it is independent on the phosphorylation of FAK.
Conclusion: When in hypoxia, this interaction occurs in chondrocytes that suffer a change in the stiffness and plays a role in the mechanisms triggered by the mechanosensing.

Keywords: Chondrocyte, Hypoxia, Mechanosensing, FAK, HIF-1α

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Author Contributions:
Patricia Sanz-Ramos – Substantial contribution to conception and design of the experiment, Data acquisition, Analysis and interpretation, Drafting the article, Final approval of the version to be published
Iñigo Izal-Azcárate – Substantial contribution to conception and design of the experiment, Data acquisition, Analysis and interpretation, Drafting the article, Final approval of the version to be published
Guarantor of submission
The corresponding author is the guarantor of submission.
Source of support
None
Conflict of interest
Authors declare no conflict of interest.
Copyright
© 2014 Patricia Sanz-Ramos et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.



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